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Reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion and hydroxyl radicals are continuously formed during normal physiological processes (e.g. mitochondrial respiratory chain) and  also by exogenous factors (e.g. toxins). Under normal conditions, the level of these species are kept checked by our antioxidant defences and hence only their important physiological roles such as in cellular/subcellular signalling is noticed. Under abnormal physiological conditions, however, the production of ROS formation  may exceed their destruction and lead to the development of pathological conditions. Cancer, ageing, Alzheimer's disease and inflammatory disorders are among the few disease conditions associated with ROS.   Natural antioxidants that either reduce the formation of ROS, those that directly neutralise ROS or those with  potential for enhancing our antioxidant defences are thus required to combat diseases mediated by  ROS. For many decades, there has also been a growing interest in finding natural antioxidants that could safely prolong the shelf-life of foods and  uses as  as nutraceuticals.

In our laboratories, we routinely  screen plant-derived potential antioxidants by using the following models:

1. Chemical assays that measure the scavenging potential of test agents against ROS.

2. Assays based on the interaction of ROS  with key biological molecules such as DNA, proteins and lipids.

3. Bioassays that measure potential antioxidant activity in a cellular system.

For any further information on the subject or  collaborative and/or consultancy work please  contact the site owner.  You may also find the following key  publications of interest:

  • HABTEMARIAM, S.*, Varghese, G.K. (2017). Antioxidant, anti-alpha-glucosidase and pancreatic beta-cell protective effects of methanolic extract of Ensete superbum Cheesm seeds. Asian Pacific Journal of Tropical Biomedicine 7(2), 121125. Abstract

  • Nabavi, S.F., HABTEMARIAM, S., Di Lorenzo, A., Sureda, A., Khanjani, S., Nabavi, S.M., Daglia, M. (2016). Post-stroke depression modulation and in vivo antioxidant activity of gallic acid and its synthetic derivatives in a murine model system. Nutrients 2016, 8(5), 248; doi:10.3390/nu8050248 (Open Access).

  • HABTEMARIAM, S., Varghese, G.K. (2015). A Novel Diterpene Skeleton: Identification of a highly aromatic, cytotoxic and antioxidant 5-methyl-10-demethyl-abietane-type diterpene from Premna serratifolia. Phytotherapy Research 29(1), 80-85.  Abstract

  • Curti, V., Capelli, E., Nabavi, S.F., HABTEMARIAM, S., Nabavi, S.M., Daglia, M. (2014). Modulation of human miR-17-3p expression by methyl 3-O-methyl gallate as explanation of its in vivo protective activities. Molecular Nutrition & Food Research 58(9), 1776-84. Abstract

  • Nabavi, F.S.,  Daglia, M.,  HABTEMARIAM, S.,  Nabavi, S.M.  (2014). Curcumin and liver health, from chemistry to medicine. Comprehensive Reviews in Food Science and Food Safety 12(1), 62-77. Abstract;  Full Text Review article.

  • Nabavi, S.M., HABTEMARIAM, S., Nabavi, S.F., Sureda, A., Moghaddam, A.H., Latifi, A.M. (2013). Prophylactic effect of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in rat’s erythrocytes in vivo. Journal of Pharmacy and Pharmacology 65(6), 868-873. .Abstract

  • Nabavi, S.F., Nabavi, S.M., HABTEMARIAM, S., Sureda, A., Moghaddam, A.H., Latifi, A.M. (2013). Neuroprotective effects of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in brain of rats. Cellular and Molecular Neurobiology 33(2), 261-267 Abstract

  • HABTEMARIAM, S.*, Sureda, A., Moghaddam, A.H., Nabavi, S.F., Nabavi, S.M., Abolhasani, F. (2013). Protective role of gallic acid isolated from Peltiphyllum peltatum against sodium fluoride-induced oxidative stress in rat’s heart. Letters in Drug Design and Discovery 10, 277-282. Abstract

  • Bose, L.V., Varghese, G.K., HABTEMARIASM, S*. (2013). Identification of acteoside as the active antioxidant principle of Premna serratifolia root wood tissues. Phytopharmacology. 4(2), 228–236.  Full Text Article

  • Nabavi, S.F., Nabavi, S.M., HABTEMARIAM, S., Sureda, A., Jafari, M., Abolhasani, F., Moghaddam, A.H. (2013). Hepatoprotective effect of gallic acid isolated from Peltiphyllum  peltatum against sodium fluoride-induced oxidative stress. Industrial Crops and Products 44, 50–55. Abstract

  • abavi, S.F., Nabavi, S.M., HABTEMARIAM, S*., Abolhasani,  F., Daglia,  M., Moghaddam, A.H. (2012). Protective effect of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in rat’s cardiac tissues. Fluoride 45(3 Pt 2), 290296.  Full text

  • Eslami S., Nabavi S.M., Latifi A.M., Mirzaei M., HABTEMARIAM, S.,  Moghaddam, A.H. (2012). Mitigating role of quercetin against sodium fluoride induced oxidative stress in rat’s brain. Pharmaceutical Biology 50(11), 1380-1383. Abstract

  • Nabavi S.M., Nabavi, S.F., HABTEMARIAM, S., Moghaddam, A.H., Latifi, A.M. (2012). Ameliorative effects of quercetin on sodium fluoride-induced oxidative stress in rat's kidney. Renal Failure    34(7), 901-906. Abstract

    Ameliorative Effects of Quercetin on Sodium Fluoride-Induced Oxidative Stress in Rat’s Kidney

    Read More:
  • Nabavi, S.F., HABTEMARIAM, S., Jafari, M, Sureda, A., Nabavi S.M. (2012). Protective role of gallic acid on sodium fluoride induced oxidative stress in rat brain. Bulletin of Environmental Contamination and Toxicology. 89(1), 73-77.  Abstract

  • HABTEMARIAM, S. (2012). Antihyperlipidemic components of Cassia auriculata aerial parts: Identification through in vitro studies. Phytotherapy Research. DOI: 10.1002/ptr.4711 Published online: 18 March 2012 Abstract

  • HABTEMARIAM, S*. and  Cowley R.A. (2012). Antioxidant and anti-α-glucosidase compounds from the rhizome of Peltiphyllum peltatum (Torr.) Engl.  Phytotherapy Research 26(11), 1656-1660. Abstract

  • Roselli, M.,  Lentini, G. and  HABTEMARIAM, S*. (2012). Phytochemical, antioxidant and anti-alpha-glucosidase activity evaluations of Bergenia cordifolia. Phytotherapy Research 26(6), 908-914 Abstract

  • Roselli, M., Lovece, A., Bruno, C., Lentini, G., Corbo, F., la Forgia F., Fontana, S., HABTEMARIAM, S., Franchini, C. (2012). Le Proprieta antiossidanti dell’uva di Troia Canosina ad acino piccolo: confronto  tra differenti metodi estrattivi. Tutto Sanita 134, 32-35.

  • Juan-Badaturuge, M.,  HABTEMARIAM, S.,  Thomas, M.J.K. (2011). Antioxidant compounds from a South Asian beverage and medicinal plant, Cassia auriculata. Food Chemistry 125(1), 221-225.  

  • Deshmukh, S.R.,  HABTEMARIAM,  S., Wadegaonkar , P.A. (2010). Antioxidant and antiproliferative activity of root suspension culture of Morinda citrifolia L. Research Journal of Pharmacy & Technology 3(4), 1189-1193. Abstract

  • HABTEMARIAM S. and Dagne E. (2010). Comparative antioxidant, prooxidant and cytotoxic activity of sigmoidin A and eriodictyol. Planta Med. 76(6), 589594. Abstract

  • Juan-Badaturugea M., HABTEMARIAM S., Jackson C., and Thomas MJK. (2009). Antioxidant principles of Tanacetum vulgare L. aerial part.  Natural Product Communications 4(11), 1561-1564. Abstract

  • HABTEMARIAM, S. and Dagne, E. (2009). Prooxidant action of knipholone anthrone: Copper dependent reactive oxygen species generation and DNA damage. Food and Chemical Toxicology47(7), 1490-1494. Abstract

  • Hevesi  B.T.,  Houghton P.J.,  HABTEMARIAM,  S.,  Kéry Á. (2009). Antioxidant and antiinflammatory effect of Epilobium parviflorum Schreb. Phytother. Res. 23(5), 719-724. Abstract

  • HABTEMARIAM, S. (2008). Activity-guided Isolation and Identification of Free Radical-scavenging Components from Ethanolic Extract of Boneset (Leaves of Eupatorium perfoliatum). Natural Product Communications 3(8), 1317-1320.

  • HABTEMARIAM, S. (2008). Activity-guided Isolation and Identification of Antioxidant Components from Ethanolic Extract of Peltiphyllum peltatum (Torr.) Engl. Natural Product Communications 3(8),  1321-1324.

  • Motlhanka, D.M.T., HABTEMARIAM, S., Houghton, P (2008).  Free radical  scavenging activity of crude extracts and 4’-O-Methyl-(-) epigallocatechin  isolated from roots of Cassine transvaalensis from Botswana. African  Journal of Biomedical Research 11, 55-63 FULL TEXT

  • HABTEMARIAM, S. (2007). Antioxidant  activity of Knipholone anthrone. Food Chemistry 102, 1042-1047.

  • HABTEMARIAM, S.  and   Jackson, C. (2007). Antioxidant and cytoprotective activity of leaves of Peltiphyllum peltatum (Torr.) Engl. Food Chemistry 105, 498–503.

  • Motlhanka. D.M.T.,  Miljkovic-Brake,  A.,  Houghton, P.J.,  HABTEMARIAM, S., Hylands, P.J. (2006). Antioxidant activity of water and  ethanol extracts from Roots of Cassine transvaalensis Burtt-Davy from  Botswana.  Planta Medica 72(11), 1001.

                 ....please also visit our publications' page for more references.


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The Site Owner

Dr Habtemariam BSc, MSc, PhD........FRSM, FRSC. .... With a Principal Lecturer post at Greenwich, Dr Habtemariam is a leader of the BSc Pharmaceutical Science programme and researches on bioassay & natural products-based drug development. ....More



In addition to our fully equipped tissue culture facilities, we have  access to various state-of-the-art equipment including ICP-MS, LC-MS, MALDI-TOF-MS, FABS-MS, FTIR, Laser-Raman,  scanning  and transmission  electron microscopes, flow cytometer, NMR (270, 300 and 500MHz),  automated DNA sequencers, various HPLC systems,  capillary electrophersis and ABI PRCmate DNA synthetisers.










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